Hi Katja,
If your protein preparation did have salt, you could try lowering that salt
(if your protein tolerates) and grid search with varying (increasing) PEG
concentrations.
Seeding and Additives should really help. the effect/outcome is difficult to
predict but these two techniques are helpful i
Hi Katja,
it makes perfect sense to add a buffer to your assay. Of course for the
beginning something which buffers well around pH 7 like HEPES, BTP, etc. would
be appropriate. If your purification protocol is optimized I would not change
the conditions of the purification buffer. But this migh
