Dear Chitra,
Crystallizing both proteins together is a very good idea: If you get a
structure, it will be an interesting one, even if the C-terminus remains
invisible.
Concerning the flexible C-terminus: is it the linker linking the second domain
to the first one? In that case it might just be
Dear Chitra,
There usually is a reason the C-terminus is disordered. Either it needs to bind
a ligand to get ordered, or it needs to bind to some other protein. You have to
check the literature. If the C-terminus binds a ligand, you have to add this
ligand to your crystallization experiment. If
