Once again, I've sent off a message only to one person that was intended for
the BB.
Begin forwarded message:
> From: "Charles W. Carter, Jr"
> Date: February 14, 2011 6:23:16 PM EST
> To: Martin Picard
> Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
>
> I tak
Is it possible that the phosphates are just disordered rather than being
cleaved? It's always the case for inactive kinase-ATP or AMPPNP complexes
that the phosphates are not stabilized by Mg2+ or the residues in the
binding pocket and hence they become disordered and are not seen in the
electron d
gt;
>
>
>
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> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Derek
> Logan
> Sent: Monday, February 14, 2011 4:16 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
>
>
>
> Hi Steve,
>
>
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Derek Logan
Sent: Monday, February 14, 2011 4:16 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
Hi Steve,
Funnily enough I just read the following paper today, which describes
exactly this phenomenon:
Hi Steve,
Funnily enough I just read the following paper today, which describes exactly
this phenomenon:
http://www.ncbi.nlm.nih.gov/pubmed/21093442
Is AMPPCP as sensitive to acid conditions? I would suspect not.
Best wishes
Derek
___
Hi Steve,
With my experience, it is (very) common to see AMPPNP is hydrolyzed to AMPPN
(supposedly) with my protein. Although the literature often reported AMPPNP
as a stable ATP mimic, such a luck wasn't true with my case, maybe same as
you. If you go to Sigma website where I purchased, it may
Hi there,
Was recently looking at a structure of an enzyme with AMP-PNP added to
the crystallization mix, and all I see is density for ADP. I was
wondering if hydrolysis of AMP-PNP to ADP is relatively common - either
as a result of extended time in crystallization or exposure of the
resultant cr
