Re: [ccp4bb] Unidentified Density

2025-01-06 Thread zeyaul islam
bacteria and purified using affinity and gel filtration chromatography. Thanks Zeya *Zeyaul Islam, PhD* QBRI - Qatar Biomedical Research Institute P.O. Box: 34110 Doha – Qatar Tel: +974 445 48033 On Mon, Jan 6, 2025 at 11:33 AM Tom Peat wrote: > Hello Zeya, > > More information may

Re: [ccp4bb] enzyme or other protein with multiple cofactors

2024-12-20 Thread zeyaul islam
Glucokinase. Single chain protein binds to no of cofactors like ATP and others apart from it's substrate glucose. Thanks Zeya On Fri, 20 Dec, 2024, 2:48 pm Harry Powell, < 193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: > Wow. I was right about one thing - this _is_ a good place to ask. >

Re: [ccp4bb] Off-topic, protein in dye-front (ion front?) on native-PAGE

2017-01-19 Thread zeyaul islam
Try Tricine gel. It is particularly suited for low molecular wt proteins and it will give you very good resolution. Even you can run it overnight at 30 V (16-18 hours). On Thu, Jan 19, 2017 at 9:33 AM, Walt wrote: > Hi, > > I have a small protein (~9 kDa) with acidic pI (~4). > When I run 18% na

Re: [ccp4bb] About protein aggregation~!

2015-05-27 Thread zeyaul islam
Hello Gaoyina S. pombe riboflavin synthase has similar situation . It is homotrimeric in solution state but the protein is monomeric in the crystal structure. Gerhardt S, Schott AK, Kairies N, Cushman M, Illarionov B, Eisenreich W, Bacher A, Huber R, Steinbacher S, Fischer M. Studies on the reacti