Hi,
In my experience, it is very very very difficult to optimize this needle like
crystal. Always give up.
Good luck!
Dear All;
Could somebody give a nice suggestion how the following type crystal could
be optimized, I almost tried everything.
Crystal Image is attached
Crystal condition: 20%
Hi,
For me it is detergent crystals or other stuff that is not quite important in
this case. As you showed the resolution is quite low. I suggest you need to
repeat again again again. You may go to PEG200. In our case, it is very
difficult to reproduce membrane protein crystals.
Good luck!
Hi,
I used 0.017% or 0.012%. My protein is very stable at this concentration.
Good luck.
Lin
2012-03-26
yybbll
Hi All,
Has anyone had any luck purifying membrane proteins with DDM
(n-dodecyl-D-maltoside) using concentration of detergent ~1-2 x CMC ? (Its CMC
is very low: 0.009
Additives screening.
YB
2011-02-21
yybbll
发件人： Bosch, Juergen
发送时间： 2011-02-21 03:46:12
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] optimization of dumbbell like crystal
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at 9
Hi, thank you for you reply. Could you tell me if you try to dye membrane
protein crystal with detergent?
Thanks.
Y.B.
2010-11-18
yybbll
发件人： Matthew Bratkowski
发送时间： 2010-11-17 02:58:40
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using
t the concentration of detergent?
And how to dilute detergent?
Thanks all.
Y.B. Lin
2010-10-04
yybbll
Hi,
A little danger this condition. I suggest you should firstly make sure they are
protein crystals. I know it is very difficult. I suggest you can add proteases
to the drops. If "crystals" dissolve, no problem, they are protein crystals.
Best
Yibin
2010-08-27
yybbll
发件人：
Hi, Did you use detergent? It seems look like detergent crystals.
2010-08-25
yybbll
发件人： rui
发送时间： 2010-08-25 20:37:18
收件人： CCP4BB
抄送：
主题： [ccp4bb] turn granular to crystal
Hi, All,
I'm trying to crystallize a soluble protein and got something like granular,
they are ro
In fact, you can put plates in refrigerator. It is cheap and simple. Maybe the
temperature is not stable, but don't warry, acturaly, the dynamic temperature
is benifit to grow crystals. You can look for relational papers.
Of course, you can buy a incubator, which can control temperature from 4-6
