Re: [ccp4bb] Substrate/Ligand Induced Oligomerization of enzymes

, 2013 at 5:03 PM, Shiva Bhowmik wrote: > Dear All, > > I am looking for references and/or example of substrate or ligand induced > oligomerization of enzymes related to activation. > > Any help in this regard would be greatly appreciated. > > Thanks. > > Shiva >

[ccp4bb] Substrate/Ligand Induced Oligomerization of enzymes

Dear All, I am looking for references and/or example of substrate or ligand induced oligomerization of enzymes related to activation. Any help in this regard would be greatly appreciated. Thanks. Shiva

[ccp4bb] Ligand Based Macromolecular Crowding or Oligomerization?

Dear All, Wish you all a very happy new year. In this new year working on a problem that I would like get your opinion. Currently working on an enzyme that turnovers Coenzyme A (CoA)-conjugated substrate. I have determined the structure of the enzyme and the structure suggests to be a single doma

Re: [ccp4bb] do you think it is interesting?

Hi Anna, Interesting assembly. What is the function of your protein? Is it known if your protein forms a fibril-like assembly in solution? Moreover, can your crystal packing be indexed higher symmetry space group? Cheers, Shiva On Mon, Jun 18, 2012 at 9:03 AM, David Schuller wrote: > If the o

[ccp4bb] Petition to Increase NIH Budget

Dear CCP4-ers, Sorry for the offline topic. I would like to bring this the following petition: Dear Colleagues, I wanted to bring your attention to a petition (see link below) started at WhiteHouse.gov by our colleague Dr. Steve Meltzer at Johns Hopkins that calls for an i

Re: [ccp4bb] Iphone app to display protein models

Dear CCP4-users, Thank you all for your suggestions. Below is the compiled list of suggestions for Iphone apps to display models: 1. Import structure directly from itunes in pdb format view in iMolview (not free). 2. Cuemol for iOS: http://itunes.apple.com/us/**app/cuemol/id496236710?ls=1&; **mt=

[ccp4bb] Iphone app to display protein models

Dear All, I was wondering if there is any IOS5 based app to display protein models, which are not in public database, on Iphone. There is an app called Molecules for displaying models but that utilizes coordinates from RCSB and pubchem. Thanks, Shiva

Re: [ccp4bb] UV imaging of crystals

Would be curious to know the current limitations on UV microscopy employed for screening protein crystals - such as content of aromatic amino acids, protein size etc. Cheers, Shiva On Fri, Sep 16, 2011 at 1:19 AM, Klaus Fütterer wrote: > From the experience when our (commercial) UV imaging syst

Re: [ccp4bb] Strange density in arginine

Hi Ruheng, Curious to know what was in the crystallization cocktail, cryoprotectant? Did you collect the dataset at a synchrotron? Shiva 2011/7/4 ruheng > Dear all, > > Recently we are working on an archaebacteria protein which was expressed > and purified from *E.coli *by conventional proced

Re: [ccp4bb] Y-Chi2 running out of chart

------ >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of >> *Shiva Bhowmik >> *Sent:* Friday, June 24, 2011 11:42 AM >> >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* Re: [ccp4bb] Y-Chi2 running out of chart >> &

Re: [ccp4bb] How to get rid of Tubulin derived from insect cell expression

Amonium sulfate precipitation? Considering the fact that you tried to seperate tubulin contamination both by charge and mass difference amonium sulfate pption may work. Shiva On Thu, Jun 23, 2011 at 4:05 AM, Seungil Han wrote: > All, > I am sorry that this is off topic. > My target protein expr

Re: [ccp4bb] Y-Chi2 running out of chart

Hi Bie, Curious to know what are the cell parameters obtained after scaling? You mention observing perfect Chi2 statistics with lysozyme crystals. But are you observing the same Chi2 statisctis with the crystal that yielded unusual Y-Chi2 if you collect another dataset. If there is a consistency o

[ccp4bb] Two different asymmetric units from two different crystallization conditions

Dear All, I am working on a protein structure that yielded comparable diffraction quality crystals from two different crystallization condition. One of the crystallization condition conatins high conc. of salt pptant whereas the oher one contains high conc. of organic pptant. There are some minor

Re: [ccp4bb] unknown density

n > > > > On May 18, 2011, at 2:03 PM, Bosch, Juergen wrote: > > > > But that's a lysine, so maybe a PTM ? Acetylated perhaps ? > > > > Jürgen > > > > On May 18, 2011, at 1:56 PM, Shiva Bhowmik wrote: > > > > Hi Cedric, > >

Re: [ccp4bb] unknown density

Hi Cedric, I presume you collected the datset at a synchrtron source. It could be free radical generated due to X-ray radiation has modified the glutamate residues. I have seen this happening for Ser residues. Cheers, Shiva On Wed, May 18, 2011 at 9:08 AM, cedric bauvois wrote: > Hi, everyone

Re: [ccp4bb] protein turns brown

That's a possibility. Do you know if the same coloration would arise if there is TCEP instead of DTT or b-ME? I usually have TCEP as the reducing agent. Thanks Fillip. Cheers, Shiva --- On Fri, 9/24/10, Filip Van Petegem wrote: From: Filip Van Petegem Subject: Re: [ccp4bb] protein turns br

Re: [ccp4bb] protein turns brown

Hi All, I also have a similar observation for proteins purified by Ni-NTA column. After concentrating the sample eluted from the Ni-NTA column, I see a brownish-yellowish tinge closer to the bottom of the filter with colorless buffer on top. This is observed even for non-metal binding and non-F