Re: [ccp4bb] Hybrid enzymes

Hi : Very interesting question that you are pointing out !. I have no experience with enzyme chimeras, however I think that if you got crystals and they diffract you should be able to solve the structure by MR using one or both structures as a seeding model within the chimera. There is a very int

[ccp4bb] BALBES : how to use a already known solution for seeding

Dear All I am using BALBES in the command line for MR. It already find a possible solution, but because of the estimated cell content, I should have at least two more monomers of my structure in the ASU. How can I fix the already known solution and use it as seed to find more ? Many thanks for y

[ccp4bb] Mosflm : data process of crystal with huge unit cell

Dear All I am processing data from a crystal for a large macromolecular complex with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222 spacegroup. To avoid overlaps, we have collected data with a oscillation of 0.1 degrees. When I try to process the data with mosflm, mosaicity decr

Re: [ccp4bb] extra line of stain in our gel

Hi Mike Sometimes keratin from your skin (fingers, nails, etc) appear in the gels as a band around 48-52 kDa. Take care in the handling of the electrophoresis should be a solution. Other possible reason could be a contamination of the sample loading buffer, also with keratin or with other protein

[ccp4bb] Arp warp 7 error in ccp4i

Dear All : When I run the new version of arp-warp in the ccp4i interface I got the following error. Any ideas that what I am doing wrong ?? Thanks for the help. Francisco - ERROR ('IOError', '', [' File "/usr/local/arp_warp_7.0/flex-wARP-src-214/CArc.py", line 78, in ch

Re: [ccp4bb] AKTA prime

Dear Frank AKTAs are excellent chromatographic systems, but you must know that AKTA prime is not the best choice if you want to do "serious" chromatography. AKTA prime has only one pump and a valve that alternatively extract solvent from the two different reservoirs. If you want to do a gradient,

Re: [ccp4bb] AKTA prime

Dear Frank AKTAs are excellent chromatographic systems, but you must know that AKTA prime is not the best choice if you want to do "serious" chromatography. AKTA prime has only one pump and a valve that alternatively extract solvent from the two different reservoirs. If you want to do a gradient,