[ccp4bb] SA-omit map

data quality is not good and twin. We tried to produce SA-omit map using phenix. The map is really bad. Does anyone have suggestion to refine the map? Thank you! Bests, zq Deng 2013-11-04 dengzq1987

[ccp4bb] off topic:how to prevent DNA tetraplexe forming?

Hi all, recently, i perform an EMSA experiment.when i prepared the DNA with a 5' poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent this happen,or has any method to disrupt the guanine tetraplexes structure? any suggestion is appreciation. dengzq

[ccp4bb] Does anyone have used DelPhi to calculate the Electrostatic potentials of DNA ？

Hi all, recently，I want to use DelPhi to calculate the Electrostatic potentials of DNA.but in the manual,i can not find the method to create the inputfile fort.11 、fort.12 and fort.13.Does anyone have experience on this? Please suggest Thank you in advance Sincerely dengzq

[ccp4bb] merge dataset

Hi CCP4bb, I have two dataset from one crystal with different distance.now i am wondering how to merge the two dataset into one file,and use it to refinement. thanks a lot! deng

[ccp4bb] improve protein-DNA complex crystal

Hi all, I got a protein-DNA complex crystal,by running SDS-PAGE and nativePAGE,i prove that the crystal contain both protein and DNA.BUT it does not diffract. does anyone have suggestion to improve the diffraction ability ? Best wishes, deng

[ccp4bb] MOLREP fails when using "input fixed model"

Dear all, Recently, I used MOLREP to molecular replacement. My OS is fedora 14 and CCP4 version is the newest one ccp4-6.2.0 . When I run a pdb file and mtz by MOLREP without input fixed model, everything is right. However, when I run it by MOLREP when using "input fixed model". The erro

[ccp4bb] screen kit

hello all, is there any screen kit that is highly effective for the crystallization of protein-nucleic acids complexes? deng.

Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

my crystal is not plate.it is Hexagonal and very large. 2011-04-05 dengzq1987 发件人： Jacob Keller 发送时间： 2011-04-05 20:53:41 收件人： CCP4BB 抄送： 主题： Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ? For plate crystals with the long axis normal to the plate surface

[ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

od to solve this problem? best wishes. 2011-04-05 dengzq1987

[ccp4bb] ssDNA self-aneal

Dear all, Recently,i purchase Oligonucleotide from company.the sequence is TTGCGTAC GCAC GTACGC .i want to perform self-annealing process to form the following second structure . 5' TTGCGTACGC ||| |||] 3' CGCATGCA i hope that most of

[ccp4bb] DNA-protein complex.

thank you! 2010-10-31 dengzq1987

[ccp4bb] ATP solution

stock solution has free pi ,maybe the ATP hydrolysis when prepared .any suggestion about preparing ATP stock solution is appreciative. Best regards! 2010-08-29 dengzq1987

[ccp4bb] Did anyone using Nano ITC low volume ( 190 ul) for protein interaction?

the standard value by CaCl2 and EDTA titration or NaHCO3 and HCl titration. Can Nano ITC low volume (190 ul) be used for measuring protein interaction? Any suggestions are very appreciated. Thanks 2010-07-23 dengzq1987

[ccp4bb] question about the zinc binding protein

protein to do zinc scan,we don't find the zinc. does anyone have some advice? 2010-03-31 dengzq1987