Dear all, recently, i prepared the ATP solution used to assay ATPase activity. when the Control Well just containing assay buffer and ATP mixed with molybdate ,the colour changed to green, this mean that there is free pi in the solution.the assay buffer and water is free from pi.and the ATP stock solution has free pi ,maybe the ATP hydrolysis when prepared .any suggestion about preparing ATP stock solution is appreciative.
Best regards! 2010-08-29 dengzq1987
