iology
Department of Biochemistry and Molecular Biology
The University of Texas Medical School at Houston
6431 Fannin Street, Houston, TX, USA, 77030
From: Clemens Vonrhein [vonrh...@globalphasing.com]
Sent: Monday, October 18, 2010 8:05 AM
To: Wu, Mousheng
Dear All,
I have a MAD dataset at 4Å and shelxD can find clear-cut 10 solutions (my
protein has 12 methionines). I ran autosharp to refine them followed by density
modification. After density modification, I ran solvent flattening. Then I
calculated the anomalous map using the phase from sharp
Hi, ccp4 community,
I am solving my protein (300 aa) structure using molecular replacement.
The space group is P622. There is only one molecule in the ASU. The
protein is supported to have three domains. We have solved the domain 1
(120aa) structure; therefore we tried to use it as a model to solv
hi, everyone!
I am struggling with my crystal structure in a very big unit cell. my protein
is about 16KDa. probably there are about 20 molecules in the asymmetric unit. I
tried to use molecular replacement to solve the structure. However, there is
only one NMR structure available for me. The
As Anthony and Jeremy suggested, I tried to fit oxalate into the
density. It perfectly matches the density.
Thank all of you. I should read more papers now to make it sense.
There are four molecules in the asymmetric unit. And this density is
present in every molecules.
-Original Message-
From: ANA MARIA MISIC [mailto:[EMAIL PROTECTED]
Sent: Thursday, July 26, 2007 5:24 PM
To: Wu, Mousheng
Subject: Re: [ccp4bb] unknown density in structure
Hi Mousheng
Hi, everyone,
I am recently solving a structure at 1.8A. After I fitted all residues
into the model, I found that there was an extra density in a pocket
consisted of E, N, R and other hydrophobic residues. The density is
strong and connected like a butterfly. I checked the crystallization
cond
