Hi, ccp4 community, I am solving my protein (300 aa) structure using molecular replacement. The space group is P622. There is only one molecule in the ASU. The protein is supported to have three domains. We have solved the domain 1 (120aa) structure; therefore we tried to use it as a model to solve the new structure. MolRep and Phaser can find the domain 1. We refined the model and the R and Rfree factor was around 50%. When we used Coot to see the model and map, we found that the other two domains are missing. In the map, there is a big 'hole' and little extra density (with 6-fold) inside. It is a big surprise to us. Is there any possibility that R is around 50 if the other two domains are missing?
Any suggests to figure out what happen to my structure? Thanks a lot! Mousheng Wu
