get some better result. I will also try to use
the Glycosylation deficient cell lines.
I am still working on it, thanks again for your valuable advice.
Best regards,
Wei
From: Matthias Zebisch [mailto:matthias.zebi...@bbz.uni-leipzig.de]
Sent: 2011年5月13日 18:15
To: Wei Li
Subject: Re
Dear all,
I am working on a high glycosylated protein, which was produced from HEK293
cells. Last time I added glycosylation inhibitor, the protein could exist some
monomers and dimers in a buffer pH5.4. This time I didn't add glycosylation
inhibitor, the protein seems highly aggregated and
