Dear all,
I am working on a high glycosylated protein, which was produced from HEK293 cells. Last time I added glycosylation inhibitor, the protein could exist some monomers and dimers in a buffer pH5.4. This time I didn't add glycosylation inhibitor, the protein seems highly aggregated and couldn't be separated in the same buffer. Does anyone have any experience working with glycoprotein and could you give me some suggestions? Many thanks in advance! Best regards, Yours sincerley, Wei [cid:image001.jpg@01CC116E.513B3050] ________________________________ Helmholtz-Zentrum f?r Infektionsforschung GmbH | Inhoffenstra?e 7 | 38124 Braunschweig | www.helmholtz-hzi.de Vorsitzende des Aufsichtsrates: MinDir'in B?rbel Brumme-Bothe, Bundesministerium f?r Bildung und Forschung Stellvertreter: MinDirig Heiko Gevers, Nieders?chsisches Ministerium f?r Wissenschaft und Kultur Gesch?ftsf?hrung: Prof. Dr. Dirk Heinz; Ulf Richter, MBA Gesellschaft mit beschr?nkter Haftung (GmbH) Sitz der Gesellschaft: Braunschweig Handelsregister: Amtsgericht Braunschweig, HRB 477
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