[ccp4bb] R: [ccp4bb] R: [ccp4bb] Heavy atom vs light atoms density

2020-06-10 Thread Vito Calderone
ishes, John Emeritus Professor John R Helliwell DSc On 10 Jun 2020, at 09:34, Vito Calderone mailto:calder...@cerm.unifi.it> > wrote: Dear Pierre, I was in particular talking of 2FoFc refinement maps. In the attached picture for example you can see the 2FoFc map contoured

[ccp4bb] Heavy atom vs light atoms density

2020-06-09 Thread Vito Calderone
aware of an article/review (to use as a reference) in which this is clearly stated/pointed out? Best regards Vito Calderone To unsubscribe from the CCP4BB list, click the following link: https

[ccp4bb] Se-Met and Se-Cys double labelling

2017-06-21 Thread Vito Calderone
I am working on a protein having 360 residues. In its sequence there are 3 Met and 5 free Cys. I will need MAD to solve the structure since based on the sequence the closest homologue has 20% identity…I suppose MR would be very unlikely to work…so I would like to express a selenium derivative to ex