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Hi Prerana, You could try 4°C as well (dont forget to use lower protein concentration) in my case this worked better. Cheers, Vicky G. Tsirkone, MSc Laboratory for Biocrystallography Department of Pharmaceutical and Pharmacological Sciences KU Leuven O&N II Herestraat 49 - box 822 3000 Leuven |

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Hi, You could change the ratio of paraffin and silicon oil (1:1 or 1:2) and/or use lower protein concentration. Best regards, Vicky G. Tsirkone, MSc Laboratory for Biocrystallography Department of Pharmaceutical and Pharmacological Sciences KU Leuven O&N II Herestraat 49 - box 822 3000 Leuven |