Re: [ccp4bb] Butandiol as cryopotectant

2010-03-23 Thread Steve Tuske
20 percent (v/v). Steve > 2,3-BD users, > > What sort of concentrations are you using? > > Dave > > -- > Delivered via an Android. > > On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote: > > Hi Ulrike, > > 2,3-butanediol has worked very nicely as

Re: [ccp4bb] Butandiol as cryopotectant

2010-03-22 Thread Steve Tuske
Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I > Hello Ulr