Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD.
It's a winner. Steve I > Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. > > Tim > > On Mon, Mar 22, 2010 at 01:32:33PM +0000, Ulrike Demmer wrote: >> Hi everybody, >> >> has anyone experience with Butandiol and its different isoforms as >> cryoprotectant ? >> >> Thanks, >> >> Ulrike > > -- > -- > Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > >
