Re: [ccp4bb] commercially available proteins

Just in case you are blocking with BSA, how about, e. coli beta-galactosidase (114 KDa) ?. It's commercially available but you probably have cells already making it in the lab On 26 April 2012 19:29, Peter Hsu wrote: > Hi all, > > Sorry for the very off topic problem. I'm looking for a loading

Re: [ccp4bb] Concentrating a protein solution - subbu

Subbu, Don't forget you crystallization screen can also be used to find a condition that your protein may be happier in ! As you already have setup a screen of your protein, have a look at your drops again and see if there are any conditions that are clear. If so, is there a consistent theme

Re: [ccp4bb] rmsd calculation for all atoms.

Michael, I think something crudely resembling what you are asking can be done with Chimera. Load your two PDBs and run matchmaker Tools > Structure Comparison > MatchMaker In the dialog box that appears, under matching, there is a box that is ticked by default "iterate by pruning long atom

Re: [ccp4bb] what package provides libg2c.so.0 on Ubuntu ? - solved

I know I'm late to the game, but for anyone who needs a supported version of libg2c.so for running old code then you need to install gcc-3.4. From the following site go to the jaunty link. You then need to install the gcc-3.4-base and the lib32g2c0 runtime library. https://launchpad.net/ubuntu/+so

Re: [ccp4bb] CCP4 / Ubuntu 9.04 64-bit

Sankar, All the executables you downloaded (including bltwish) from the CCP4 site are compiled on a 32bit machine. You just need to install the 32bit shared library package via synaptic. The following terminal command should do. sudo apt-get ia32-libs As for coot for what platform is your v

Re: [ccp4bb] CCP4 / Ubuntu 9.04 64-bit

Just to add to this post, we've been successfully running Coot on 64 bit Ubuntu 9.04 using the X86 Centos-5 stable build (0.52) from Paul Emsleys's site: http://www.ysbl.york.ac.uk/~emsley/software/binaries/stable/ The only quirk I have found so far is that the window will flicker on and off whe

Re: [ccp4bb] Shredded E coli pellets

Jacob Before you spend you holiday weekend "dephaging" the lab and picking up on a comment from Artem and yourself, your protein could indeed be toxic to the cells. You do state in your email that the your are working with new constructs. What happens with your old constructs ? I've been unfortunat

Re: [ccp4bb] How to express a >95KD FAD protein

presence of FAD may work. A quick search in the journal Protein Expression and Purification suggests the latter has been done a few times. Stephen Weeks 2009/6/29 Yong, Wei > Dear all, > > I know that there are a lot of experts having experience in expressing a > big protein in E.coli

Re: [ccp4bb] Off topic: Mammalian gene expression in E. coli

the amino terminus was highly ordered in the structure (I could use the Selenium labeled start methionine for phasing). I'm curious if this can be extrapolated to all poorly cleavable fusion constructs. Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of B

Re: [ccp4bb] Off topic: Mammalian gene expression in E. coli

all who responded. Actually, this bulletin board is better for help with molecular biology than the molecular biology bulletin board I am subscribed to! On Tue, Feb 24, 2009 at 7:47 PM, Stephen Weeks wrote: Mo, Just to add my 50 cents, I didn't see any mention of the

Re: [ccp4bb] Off topic: Mammalian gene expression in E. coli

low levels. Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of Biochemistry and Molecular Biology Room 10102 New College Building 245 N. 15th St. Philadelphia, PA 19102 Phone: (+) 215-762-7316 Fax: (+) 215-762-4452 Mo Wong wrote: I thought I'd post thi

Re: [ccp4bb] Offtopic-ish: Ubuntu Laptop

using them almost exclusively for crystallography for many years now. James On Feb 19, 2009, at 7:47 PM, Stephen Weeks wrote: Dear BBers, I would like to treat myself to a new laptop which will be my primary use machine (i.e I want to run all the usual crystallography packages, hopefully write a

[ccp4bb] Offtopic-ish: Ubuntu Laptop

tinkering around a bit to get things to work as that's part of the fun. Cheers Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of Biochemistry and Molecular Biology Room 10102 New College Building 245 N. 15th St. Philadelphia, PA 19102 Phone: (+) 215-762-73

Re: [ccp4bb] Removal of glycosylation sites in Picha expression construct

) specifically at the site of glycosylation. Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of Biochemistry and Molecular Biology Room 10102 New College Building 245 N. 15th St. Philadelphia, PA 19102 Phone: (+) 215-762-7316 Fax: (+) 215-762-4452

Re: [ccp4bb] Removal of glycosylation sites in Picha expression construct

ion site to an Alanine appears to work. I also agree with others on the CCP4 bb of trying the mutations individually. Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of Biochemistry and Molecular Biology Room 10102 New College Building 245 N. 15th St. Philadelp

Re: [ccp4bb] Off topic: General rule for maximum flow rate for affinity column?

shing and elution I usually carry these out at the same flowrate as loading (including GST resins). Stephen -- Stephen Weeks, Ph. D. Drexel University College of Medicine Department of Biochemistry and Molecular Biology Room 10102 New College Building 245 N. 15th St. Philadelphia, PA 19102