Dear Eugene
Thanks for sharing the sad news. Alexey was a wonderful person and huge
help for setting up our MorDA-powered Contaminer server.
a big loss for the community!
Stefan
Stefan T Arold,
Professor of Bioscience
Associate Dean, Division of Biological and Environmental Sciences and
Engin
Dear Julian
thank you for pointing out that our server had problems! We have now fixed
them and jobs should run as usual. Please let me know if any problems
persist.
Thanks for using ContaMiner!
With best wishes
Stefan
On Sat, 4 Dec 2021 at 21:27, Julian Nomme wrote:
> Hi all,
>
> I sent several
Dear Colleagues,
If you are looking for a Post-Doc position in place where you don’t have to
wait for weeks to measure on a TITAN KRIOS equipped with a direct detector
and phase plate, then please consider the position that I have currently
available in my group for an EM-savvy structural biolog
Dear Colleagues,
If you are looking for a place where you don’t have to wait for weeks to
measure on a TITAN KRIOS equipped with a direct detector and phase plate,
then please consider our opening for EM-savvy faculty at KAUST.
KAUST, founded in 2009, is located at the shores of the Red Sea i
is absolutely right, it would
> >> have
> >> > >>>> been
> >> > >>>> nice to raise such issues first with the developers. In my
> >> experience,
> >> > >>>> Staraniso does a fantastic job if used correctly.
&
uld only teach people to do their job (or train their robots)
> properly.
> > >>>>
> > >>>> Best wishes,
> > >>>>
> > >>>> Radu
> > >>>>
> > >>>> --
> > >>>&g
Dear Community,
A quick message to announce the following two new features on our
ContaMiner web server for the automated detection of unwantedly
crystallised contaminants (
https://strube.cbrc.kaust.edu.sa/contaminer/submit)
1) online visualisation of 2FoFc and FoFc maps. In cases of positive
re
Hi Mohamed,
I don't recall such a case of a crystallized proteolytically cleaved
contaminant (see our recent summary of contamination cases
http://scripts.iucr.org/cgi-bin/paper?ei5009 , and references therein).
I would certainly recommend depositing the data at the PDB - it may help
others address
Required skills*: Experience in protein expression, purification and
characterization; Expertise in structural methods, in particular cryo-EM
and X-ray crystallography. A high level of motivation and the ability to
work as part of a team are essential.
*Location:* Group of Stefan Arold at th
Dear Crystallographers,
Just a quick message to thank all those who have contributed suggestions
and protein contaminants for our protein contaminant *ContaMiner &
ContaBase* web server and database (
https://strube.cbrc.kaust.edu.sa/contaminer).
Thanks to this community effort we have now 65 con
Dear Myeongseon,
my favourites are
SwissModel (especially good for modelling close homologues, since it keeps
conserved side chains & backbone as in the template; very fast (5-50 min),
nice interactive user interface)
RaptorX (good for more remote homologues too, also gives you secondary
structure
US elections, UK BREXIT, Colombia referendum . . . if you are fed up with
democracy, consider kingdoms. Middle East is still investing into science,
technology and education!
Cheers,
Stefan
On 9 November 2016 at 16:22, Noam Adir wrote:
> Dear Ian,
>
>
>
> I have always thought that this saying
Dear All,
I am looking for a sealed tube in-house X-ray system that can (almost
literally) withstand sandstorms. The system should be largely
maintenance-free, robust to infrequent use and humid climate, with
intuitive and simple piloting software. It should allow both, rapid
screening and collecti
Dear Alison,
Christian Dumas of our institute has recently developed and published a
program for HA detection. Being extremely powerful, and easy to use
(http://www.cbs.cnrs.fr/SP/crystal/SUPERFLIP/), it has produced very precise
HA positions for datasets that no other program could treat previou
Dear Miriam
I had that case of the 4-helix bundle FAT domain of Focal Adhesion
Kinase. It crystallised as a helix-swapped dimer (PDB ID 1k04,
1mol/ASU), as a non-swapped 4helix bundle (1ow7, 3 mol/ASU), and as a
hybrid, where 2 mols / ASU were not swapped, and one was swapped (1ow6).
With be
arent appropriate here and may be
muddling things up.
On the other hand you shouldnt get any repulsion between pairs of
atoms whose added occupancy is <= to 1.0
Is that always the case for your 2 scenarios?
Try removing the A and B flags and see if that is better.
Eleanor
Stefan Arold wrote
Dear All,
I am having trouble refining a difficult case of alternate structures in
REFMAC. In the crystal, two states superimpose:
1) Apo-protein with its own auto-inhibitory tail fixed into the ligand
binding site
and
2) Peptide-bound protein, where the peptide has displaced the
auto-inhibit
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