[ccp4bb] Exonuclease resistant ssRNA

Dear all, I am trying to reconstitute a 5' to 3' exonuclease bound to substrate RNA for structural studies. For this, I would like to synthesize a 40nt ssRNA containing a modified residue will inhibit the exonuclease activity eg: at position 20. I could find from the literature that using phosph

[ccp4bb] Amphipols for soluble protein stability

Dear all, I am working with a multi-subunit complex which needs a minimum of 5% glycerol for stability in solution. I usually reconstitute this complex together with multiple interaction factors as a part of big ensemble over GraFix for cryoEM grid preparation in a final buffer co

[ccp4bb] Sokalan CP5 and CP7

Dear all,     I recently had a few initial hits for my protein in conditions with Sokalan CP5 and CP7 (Midas Screen). There is not much on the web about these compounds except for a thread on CCP4bb last year which doesn't include information about the suppliers.  The standard suppliers (si

[ccp4bb] off-topic: protein ligation and phospho-tyrosine affinity chromatography

Dear all,     We are planning to ligate a phospho-tyrosine containing 16aa/33aa peptide onto a 70 kDa protein and subsequently use it for crystallography studies. We will be using the Intein-fusion protein system (IMPACT kit from NEB) to accomplish the ligation. The idea is to separate the