Dear all, I am trying to reconstitute a 5' to 3' exonuclease bound to substrate RNA for structural studies. For this, I would like to synthesize a 40nt ssRNA containing a modified residue will inhibit the exonuclease activity eg: at position 20. I could find from the literature that using phosphorothioate or thiophosphate, it can be achieved.
But, it would be very helpful to know if someone has a working strategy to arrest an exonuclease in this way and could share from where we can get such RNA synthesized. Many thanks in advance for all your inputs. Kind regards,Srinivasan ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
