on. Checking at diff. pH buffer or temperature can also refine
> your data to a great extent.
>
> good luck
>
>
> On Mon, Aug 4, 2014 at 12:14 PM, Sonia Majumdar
> wrote:
>
>> Dear all,
>> I am working on a GTPase with two tandem G-domains. While doing ITC
>
Dear all,
I am working on a GTPase with two tandem G-domains. While doing ITC
experiments the heat change obtained is very poor 0.1ucal/sec, however the
ITC profile is showing gradual saturation. The n value is very poor 0.33.
I presumed the protein has bound GDP and tried to separate the nucleoti
