Dear all,
I am working on a GTPase with two tandem G-domains. While doing ITC
experiments the heat change obtained is very poor 0.1ucal/sec, however the
ITC profile is showing gradual saturation. The n value is very poor 0.33.
I presumed the protein has bound GDP and tried to separate the nucleotide
bound and unbound forms using Mono-Q. 3 peaks were obtained containing the
same protein. However, ITC done with the proteins corresponding to the 3
peaks gave the same results.
Please suggest what could the three peaks possibly mean and how to modify
the ITC experiments.
Thanks in advance.
Regards.
