Re: [ccp4bb] About protein aggregation~!

2015-05-27 Thread Sebastiaan Werten
species in solution is very low. In spite of this, well-documented cases and/or systematic studies in the literature seem to be fairly rare. Below is a list of replies with relevant cases. HTH, best wishes, Sebastiaan Werten. Luca Jovine: Sure - crystallization selects what packs, which may constit

Re: [ccp4bb] Crystallisation of a minority fraction monomers

2015-04-08 Thread Sebastiaan Werten
 http://www.ebi.ac.uk/pdbe/pisa/ best regards Marjolein Thunnissen On 08 Apr 2015, at 15:07, Sebastiaan Werten wrote: Dear all, we are currently working on a protein that is known to exist in a monomer-dimer equilibrium. At the high concentrations used for crystallisation assays, the dimer is predom

[ccp4bb] Crystallisation of a minority fraction monomers

2015-04-08 Thread Sebastiaan Werten
ishes, Sebastiaan Werten.

Re: [ccp4bb] Additional band on gel due to his-tag: any references?

2010-10-28 Thread Sebastiaan Werten
terature there either. Seb. -- Dr. Sebastiaan Werten Institut für Biochemie Universität Greifswald Felix-Hausdorff-Str. 4 D-17489 Greifswald Germany Tel: +49 38 34 86 44 61 E-mail: sebastiaan.wer...@uni-greifswald.de On Thu, 28 Oct 2010 10:09:37 -0400, "Skrzypczak-Jankun, Ewa" wrote: &g

[ccp4bb] Additional band on gel due to his-tag: any references?

2010-10-28 Thread Sebastiaan Werten
additional band is a very common artifact with his-tagged proteins, but I was wondering if anyone is aware of a paper that formally describes the phenomenon, as we need to appease a couple of rather bloody-minded referees. Thanks very much for any suggestions, Seb. -- Dr. Sebastiaan Werten Institut