Dear all,
we have a his-tagged protein that shows a minor accompanying band in
SDS-PAGE, just above the main band. According to all other methods
available to us the material is homogeneous, the protein has the
correct
mass in MALDI-TOF, epitopes are recognized, etc. etc.
I know that the additional band is a very common artifact with
his-tagged proteins, but I was wondering if anyone is aware of a paper
that formally describes the phenomenon, as we need to appease a couple
of rather bloody-minded referees.
Thanks very much for any suggestions, Seb.
--
Dr. Sebastiaan Werten
Institut für Biochemie
Universität Greifswald
Felix-Hausdorff-Str. 4
D-17489 Greifswald
Germany
Tel: +49 38 34 86 44 61
E-mail: sebastiaan.wer...@uni-greifswald.de
