Re: [ccp4bb] How to model apo protein structure from solved ligand bound high resolution structure?

Hi Donghui, It’s going to be tricky. Perhaps, you can predict some local rotamer variations around the ligand interacting regions. Predicting the global changes is difficult, particularly if you are anticipating a big conformational change. For example, in one of our structure, the active s

[ccp4bb] purification of phosphorylated proteins-off topic

Dear CCP4BB, This is an off topic question for CCP4BB, I am posting on behalf of my friend: Hi, I am trying to find a method to purify/separate phosphorylated protein from unpshosphorylated proteins. I have two approach in my mind ion-exchange Fe columns, I tried this too but limited