Re: [ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

<1 sigma. If you look > at the Fo-Fc map, there are large areas of positive and negative density. > The 2mFo-Fc map looks very convincing, you can see the tyrosines, phenyl > rings, etc...Also phaser gave LLG of >400 and a TFZ of ~14. Rfree is ~0.45. > Does anyone know what's

[ccp4bb] Fwd: [ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

ome Trust/MRC Building Fax: +44 1223 336827 > Hills Road > E-mail: rj...@cam.ac.uk > Cambridge CB2 0XY, U.K. > www-structmed.cimr.cam.ac.uk > > > On 26 Apr 2017, at 02:23, Roger Shek wrote: > > > > Hi everyone, > > > > We hav

[ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

ma. If you look at the Fo-Fc map, there are large areas of positive and negative density. The 2mFo-Fc map looks very convincing, you can see the tyrosines, phenyl rings, etc...Also phaser gave LLG of >400 and a TFZ of ~14. Rfree is ~0.45. Does anyone know what's going on? Thanks, Roger

[ccp4bb] Coot Find Ligand Places Ligand In Density Outside Where Model Is Built

not where the cursor was? I tried renaming the chains, but now it the find ligand doesn't even find anything even though there is clear density there. Thanks in advance. Roger -- Roger Shek Stony Brook University Graduate Student in Biochemistry and Structural Biology (PhD) Stony Brook Integr