Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-13 Thread Roessler, Christian
Another thing to try if you want to "speed up" the soaking process is to optimize your crystal growth to give smaller crystals. 10-20 micron crystals can reach 80% occupancy in less than 30 seconds, at least in our very limited tests. But this quick soak method was important for our system as

Re: [ccp4bb] Resuspension of bacterial cell pellets

2012-10-26 Thread Roessler, Christian
This is the heating information found on BioSpec's website: Like many mechanical cell disruption techniques, the Beadbeating process generates heat. In general for every minute of beadbeating there is a 10 deg rise in sample temperature. Should that be a problem, the ice-water jacket included w