Re: [ccp4bb] Best (Suitable) Mac Laptop configuration for protein Xtallography

2015-04-02 Thread Rob Gruninger
Keep in mind the updated 15" MacBook Pro is slated to come out soon (sounds like early summer). Probably won't have more RAM but is supposed to have a new processor. If you can hold off for a couple months I would suggest waiting as these haven't been updated in almost a year. Rob On Thu, Apr 2,

[ccp4bb] Off Topic:crystallization in the presence of glycerol

2007-08-24 Thread Rob Gruninger
Dear All, I am working with a protein that requires 10% glycerol throughout the purification to keep it soluble. I have been very worried that having glycerol in my protein solution when I am trying to crystallize it will prevent me from obtaining crystals. I am curious to see if others have succes

Re: [ccp4bb] crystal with precipitation

2007-08-23 Thread Rob Gruninger
Hi Shivesh I had something like this recently and found that changing the pH stopped the precipitation and the size of the crystals improved. If the crystals are large enough even if there is ppt present I would still try and collect data on them. This is likely not the case for you or you wouldn