Re: [ccp4bb] SeMet protein behaves much different with native protein..

Dear Benini,Tina, Tim, Patrick, Tom, James, Mark and other CCP4ers, Thank you very much for your suggestions! First, as you suggested, I already applied microseeding (strategies: seeds vs precipitants; seeds vs drop ratio) . SeMet protein indeed crystallized in that case, but crysta

[ccp4bb] SeMet protein behaves much different with native protein..

Hi, I'm now facing a problem about selenomethionine-labelled protein crystallization.. Native protein crystallized very well and diffracted to 2.5A. However, SeMet-protein expression level decreased to only 1/10, and its crystals have different shape with before(native protein crystals have b