My company is currently hiring and I thought there would be some good overlap
of skills with the talented people on this mailing list. At LabGenius, we’re
building a platform to discover revolutionary biological medicines coupling
wet-lab techniques with machine learning and automation. We have
These are not structural biology roles, but I thought there would be some
overlap with the skills of people on this forum. We are looking for innovative
people to join our startup company, LabGenius. We engineer proteins with both
enhanced and entirely novel functionality with the use of machine
I am interested in purifying proteins with 1 or 2 disulphide bonds, and have
been using enterokinase to cleave off N-terminal tags but we have had issues
with poor cleavage and want to try TEV cleavage instead. However TEV protease
is usually kept in a high amount of DTT and I am concerned about
From a fluorescence scan it would appear a protein I am working on has zinc in
it. The occupancy is likely to be very low however (a structural homologue has
several zincs in the x-ray crystal data but at 0.5 occupancy), as there isn't
anything obvious in the electron density map (perhaps some o
A while back I was shown in Coot how to take a ligand from one PDB and insert
it into another one - this is really useful if you have superimposed a
homologue and the ligand is in the perfect position. However I have forgotten
how to do this! Could someone please remind me?
Thanks in advance fo
I have recently solved a novel structure which previously did not have any
structural homologues (as related by sequence identity). I was wondering if
anyone could recommend a 3D structural search engine? I used the Dali server
which has been recommended to me in the past (and with past success)
Hello all,
I am intending to do selenomethionine labelling in E.coli, however my
department have asked me for a risk assessment for the experiment due to the
toxicity of the selenomethionine. It would be a great help to me if anyone out
there already has one that they could email to me to look
Hello all, I have heard at several CCP4 meetings and also at Diamond training
that a good "cut off" for CC(1/2) is around 0.3 and I/sig(I) is 0.2, but I am
struggling to find any journal references to say this (other than demonstrating
the merits of CC(1/2) over Rmeas).
Can anyone point me in
