Re: [ccp4bb] Create a dual boot windows-linux machine

Hi Xie, My way to do this work is: 1. Create the swap partition (4Gb) as logical one 2. Then create another Ext3 partition (taking the rest of free space) as logical one 3. When install the Linux, mount Swap partition as Swap and Ext3 partition as / That's all you need to consider. You don't need

[ccp4bb] Poll Result: Monoclonal Antibody for Membrane Protein

Dear Scientists, I am happy to receive your helpful support to give the raw overview of this problem. Here I would like to give you the information so far. The question is: "Have you ever applied monoclonal antibody for crystallization of membrane protein?" *Total voter: 48* 26 people (*54%*) are

[ccp4bb] Poll: Monoclonal Antibody for Membrane Protein Crystallization

Dear Crystallography Scientists, We are working on some membrane proteins and would like to apply monoclonal antibody to facilitate the crystallization. Even this method showed very good results in some cases, I am really not sure about other failure cases that are not reported. Because this meth

[ccp4bb] SUMARY: Optimization of needle crystals

Dear ccp4 Users, I would like to say thank you to all of your valuable advices to optimize the needle crystal and related problems when dealing with membrane protein. Here I report the summary of your advices to help other students who got the same problem: 1. Additive screening from Hampton Rese

[ccp4bb] protein degraded when setting xtal?

Dear CCP4 experts, I'm working on a kind of cystein peptidase. It shows a little degradation after 2 days storing at 4 degree even I tried many kinds of protease inhibitors, or glycerol and DTT. So I decided to work fast (1 day) then set xtal. After one week I pick up some condition and check degra

Re: [ccp4bb] crashing-out protein eluted from Nickel column

Hi, I used another way to deal with this problem. You can try to elute your protein with the buffer containing 50mM EDTA (You need at least 10CV to elute completely). Then use gel filtration to remove the Ni. I applied this method with two proteins and it showed good results. Good luck! TriNgo --

[ccp4bb] Expired Crystallization Screening?

Dear CCP4 users, I'm sorry for non-ccp4 question. My lab is using protein crystalization screening from Hampton and Emerald. However I noticed the Expires Date was 05/2002. That means we are using the too old chemical. Could I ask your experience about this problem? Should I need to remove these ex

[ccp4bb] Question regards to binding affinity of the protein complex?

Dear CCP4 Users, I'd like to solve the structure of the protein-protein complex. I intend to purify and incubate the complex then run gel-filtration before setting crystal. I'd like to know your experience about the Kd value of the interaction in order to get the complex after running the gel-filt

Re: [ccp4bb] J. Struct. Biology EndNote style

Dear Mr. Artem, I think you can download this style from http://www.endnote.com/support/enstyledetail.asp?SORT=2&PAGE=1&METH=0&DISC=none&JOUR=structural&BSRT=none&FF1=none&FF2=none&FF3=none&CITE=none&DKEY=714200664522dAA%20%20%20%20%20%20%20%20B There are other styles for many kinds of journal. I

[ccp4bb] SUMMARY: Cannot run NTA to purify the protein having Histag?

Dear CCP4 users, Thank you all of your advices which help me to solve this problem. I believe that all of your advices will help me and others having the same problem. This is the summary of experienced advices about how you will do if the protein having His-tag don't bind to NTA resin. 1. The pr

[ccp4bb] Cannot running NTA to purify the protein having His-tag?

Dear CCP4 users, I'm purifying a kind of protease having His-tag. The protein is expressed in insect cells and broken by sonication. I used NTA resin to purify this protein. Buffer A is 50mM phosphate buffer pH 7.5 and 300mM NaCl. Buffer B is 50mM phosphate buffer pH 7.5, 300 mM NaCl and 300 mM I