Dear CCP4 Users, I'd like to solve the structure of the protein-protein complex. I intend to purify and incubate the complex then run gel-filtration before setting crystal. I'd like to know your experience about the Kd value of the interaction in order to get the complex after running the gel-filtration. In other words, what is the lowest Kd that we can purify the protein complex using gel-filtration? And if the binding affinity is too low to purify the complex, is it possible to get the crystal of the complex if I just incubate and set crystal of this mixture?
Thank you very much for all of your advice! My best regards, TriNgo Sungkyunkwan University
