Hi Marco,
Thank you for the reply and this information.
I liked the idea of using this plasmid for all three expression systems. We
will check the virus titer before infecting insect cells for optimum protein
expression. I have not tried the neomycin version of the plasmid, but I will,
too.
T
Dear all,
I just cloned a large gene into pTriEx plasmid to attempt protein expression in
bacteria, insect cells and mammalian culture.
I know that this plasmid works well for protein over-expression in bacterial
system but I have not yet tried it with baculovirus system.
I wonder if someon
Dear all,
We have a 4-year BBSRC-funded multi-disciplinary postdoc position in the
Laboratory of Dr Mark Shepherd, University of Kent.
As it is a multidisciplinary project, three project co-leads, Dr Dave Beal, Dr
Gary Robinson, and I (Dr Mohinder Pal), are also based at Kent University
Dear CCP4 members,
My group is expanding at the University of Kent, and we have a three-year
postdoc position available in my laboratory.
We seek an enthusiastic individual interested in structural biology, especially
single-particle cryo-electron microscopy and X-ray crystallography technique
> On 17 Jul 2024, at 19:14, Mohinder Pal wrote:
>
> Dear CCP4BB members,
>
> I am delighted to share that a three-year postdoc position is available in my
> group. We are looking for an enthusiastic individual interested in
> biochemistry and structural biology, especially
enquiries, please contact Mohinder Pal at
m@kent.ac.uk <mailto:m@kent.ac.uk>.
For more details and to apply for this position, please follow the link below:
https://www.jobs.ac.uk/job/DIT588/research-associate.
Best wishes
Mo
ishes,
Mohinder Pal
--
"Whatever you’re meant to do, do it now. The conditions are always impossible.”
Doris Lessing
--
> On 18 Apr 2019, at 07:36, Bärbel Blaum wrote:
>
> Hi Mohinder,
>
>
for
better resolution as I plan to add more components to this existing complex.
Best wishes,
Mohinder Pal
--
"Whatever you’re meant to do, do it now. The conditions are always impossible.”
Doris Le
Dear Liuqing,
It could be good to dissolve the crystals to check if the crystallised protein
is still intact. If it is truncated then make a new construct based on
truncation. It could improve the crystal diffraction as it was the case for me.
Best wishes,
Mohinder
--
Dear Herman,
I have crystallised the same protein with 6xHis and 12xHis and they both
diffracted to high resolution. However, I have not seen these tags in the
structure.
Best wishes,
Mohinder
Sent from my iPhone
> On 19 Sep 2017, at 12:10, herman.schreu...@sanofi.com wrote:
>
> Dear BB,
>
structure but I am just curious to know one
restrain equals how many observations.
I look forward to hear your suggestions.
Kind regards,
Mohinder Pal
Thanks a lot to all the CCP4 members for help. The problem is solved now and
I can run CCP4 programmes on mac.
Regards,
Mohinder
Dear ccp4bb,
I have just installed ccp4 6.1.2 with ccp4i 1.4.2 on a MacBook Pro running OSX
10.5.8.
When I try to run Refmac5 (or phaser) I get the status "starting" in the GUI
but the program
fails to run and no log file is generated. I source:
/usr/local/ccp4-6.1.2/bin/ccp4.setup-sh
befor
Hi!
I have recently bought MacBook Pro and I have specific problem with installing
coot on it. I
already followed Scott lab's instructions but the problem still exists. All
help and
suggestions are most welcomed.
Cheers!
Mohinder
Hi
I am currently refining a structure of glycoprotein with drug bound to it
a. I refined the protein structure with drug using Refmac5 and now I want to
add the sugar molecules. I dont know how to combine the two cif files
(drug.cif and sugar.cif) together so that I could refine the structure wi
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