Dear Herman, I have crystallised the same protein with 6xHis and 12xHis and they both diffracted to high resolution. However, I have not seen these tags in the structure.
Best wishes, Mohinder Sent from my iPhone > On 19 Sep 2017, at 12:10, herman.schreu...@sanofi.com wrote: > > Dear BB, > > We are planning the production of a protein for crystallization. From > literature, we know that the construct with a 6-histidine tag crystallizes. > However, for other biophysical measurements, we would prefer to have a > 10-histidine tag. > > Does anyone has experience with His-6 versus His-10 tags in terms of > crystallization success? > > Thanks for your help! > Herman > >
