Re: [ccp4bb] freezing in ethane / propane - unipucks ?

2022-01-27 Thread Minmin
the CombiPuck transport canister easier from the shipping dewar at synchrotron because one hole and a groove on the puck pusher may align with two rods on the canister to get two rods squeezed to pull the canister out.  Minmin On Wednesday, January 26, 2022, 5:26 pm, Guenter Fritz wrote

Re: [ccp4bb] High-order oligomers vs robust crystals - references?

2021-12-01 Thread Minmin
ric ensemble might first form transiently in solution and then be incorporated into the crystal via surface patches introduced by mutagenesis. A similar mechanism is observed for other proteins crystallized by this approach (Z. S. Derewenda, unpublished).” Minmin On Wednesday, December 1, 2

Re: [ccp4bb] High-order oligomers vs robust crystals - references?

2021-11-15 Thread Minmin
naged to crystallize successfully and solve the structures over a decade ago. The oligomer interactions were shown in the structures.  Minmin On Sat, 13 Nov 2021 at 13:31, Amir Khan wrote: Intuitively, when jagged and oddly-shaped proteins oligomerize, it would make sense they coul

Re: [ccp4bb] Stabilizing Mitegen reusable bases/mounts

2020-11-16 Thread Minmin
When trying to recover the bases glued with the pins, if happening the acetone possibly to get bases rusty, the bunsen burner flame may help melt the glue to get pins removed from the bases quickly and cleanly. Of course, be cautious about the fire safety and use forceps to hold the pins during

Re: [ccp4bb] dewar horror stories

2020-06-26 Thread Minmin
A collaborated group met the similar situation with FedEx before and they switched to World courier afterwards.  When your shipping dewar is no longer leaking of course, maybe you would like to check out the following link below. According to our users, crystals stored in the CombiPuck seem to g

Re: [ccp4bb] challenges in structural biology

2019-07-15 Thread Minmin Yu
Hi James,  7) or 8) What are a few different collection methods including at RT for serial crystallography data? Temperature vs global and site-specific radiation damage  --- RT serial crystallography             Temperature vs multiple conformers    Thanks, Minmin   > Dear James, > >

Re: [ccp4bb] challenges in structural biology

2019-07-15 Thread Minmin Yu
Hi James,  7) or 8) What are a few different collection methods including at RT for serial crystallography data? Temperature vs global and site-specific radiation damage  --- RT serial crystallography             Temperature vs multiple conformers    Thanks, Minmin   > Dear James, > >

Re: [ccp4bb] challenges in structural biology

2019-07-15 Thread Minmin Yu
Hi James,  7) or 8) What are a few different collection methods including at RT for serial crystallography data? Temperature vs global and site-specific radiation damage  --- RT serial crystallography             Temperature vs multiple conformers    Thanks, Minmin   > Dear James, > >

Re: [ccp4bb] challenges in structural biology

2019-07-15 Thread Minmin Yu
Hi James,  7) or 8) What are a few different collection methods including at RT for serial crystallography data? Temperature vs global and site-specific radiation damage  --- RT serial crystallography             Temperature vs multiple conformers    Thanks, Minmin   > Dear James, > >

Re: [ccp4bb] challenges in structural biology

2019-07-15 Thread Minmin Yu
Hi James,  7) or 8) What are a few different collection methods including at RT for serial crystallography data? Temperature vs global and site-specific radiation damage  --- RT serial crystallography             Temperature vs multiple conformers    Thanks, Minmin   > Dear James, > >

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Minmin Yu
@JISCMAIL.AC.UKReply To: Frank Von DelftSubject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Or do sitting drop, it's much easier all round. Frank Sent from tiny silly touch screen From: Minmin Yu Sent: Thursday, 31 January 2019 21:42 To: C

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Minmin Yu
sitting drops sealing with grease. Minmin > On 31 Jan 2019, at 21:14, Daniel M. Himmel, Ph. D. > wrote: > > Fisher Scientific makes 22 inch square plastic cover slips, which are labeled > "Fisherbrand Unbreakable > Cover Slips". > > I and my colleagues have u

Re: [ccp4bb] Looking for alternatives for discontinued crystallography items

2018-10-12 Thread Minmin Yu
e X-ray beam probably due to vial protection of the crystals with liquid LN2 to hold longer time of LN2 temperature to the crystal samples. Minmin > On 12 Oct 2018, at 09:36, Alice Dawson (Staff) > wrote: > > Hi Matthew > > In the UK Molecular Dimensions sell the cane s