When trying to recover the bases glued with the pins, if happening the acetone
possibly to get bases rusty, the bunsen burner flame may help melt the glue to
get pins removed from the bases quickly and cleanly. Of course, be cautious
about the fire safety and use forceps to hold the pins during the process, not
to pointing the pins to oneself or anybody else although the pins usually won't
be shot out too far.
So the reuseable pins can be glued if worrying about the shifting for the bases
can be recovered OK afterwards.
On Mon, 16 Nov 2020 at 16:51, Diana
Tomchick<diana.tomch...@utsouthwestern.edu> wrote: #yiv9535150920 P
{margin-top:0;margin-bottom:0;}We no longer use the reusable bases for the same
reasons, but we do re-use our bases. If you use superglue to glue the pins into
the bases, you can remove the pins from the bases by soaking them in acetone.
This works about 75% of the time, especially if you are careful not to use too
much superglue when you glue the pin into the base.
It goes without saying that the use of acetone will dissolve the loop, but then
the main reason we remove the pin is due to imperfections in the loop, so no
big deal.
Whenever we see the motions that you describe, we postulate that somehow the
pins were damaged and they are loose in the base. Occasionally this motion is
ascribed to ice between the base of the pin and the magnetic mount, but this
happens very rarely in our experience.
Diana
**************************************************
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX 75390-8816
diana.tomch...@utsouthwestern.edu
(214) 645-6383 (phone)
(214) 645-6353 (fax)From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf
of Tao-Hsin Chang <taohsin.ch...@gmail.com>
Sent: Sunday, November 15, 2020 3:07 PM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Stabilizing Mitegen reusable bases/mounts
EXTERNAL MAIL
Hi Patrick,
I have met the same issue and that is very bad for any micro-focus beam and
small crystals. I have talked with Mitegen and they are developing a new type
of reusable base having an improvement for this issue. You may get some update
from them. But, I do not use the reusable bases anymore.
Best wishes,Tao-Hsin
On Nov 15, 2020, at 3:45 PM, Patrick Loll <pjl...@gmail.com> wrote:
Hi everyone,
I’ve become very fond of the Mitegen reusable bases for mounting crystals,
since the reusable aspect savse me from having to discard the base every time I
break a microloop. However, once the crystals arrive at the synchroteon, I
observe motions of the loops (some gradual, some sporadic). The the amplitudes
of these motions are becoming significant as I take data from smaller and
smaller crystals. I don’t think I’m imagining this, since the good folks at
NSLS-2/AMX have warned me about this very issue.
I’m writing to ask if anyone has any clever ideas about stabilizing these
assemblies. Obviously, I can epoxy the pins in place, but then I’ll probably
need to discard the entire assembly when I break a loop, and I’d prefer not to
waste more money than necessary. I’ve considered putting a bead of wax at the
point where the pin enters the base (although I haven’t yet checked to see if
that will survive immersion in liquid nitrogen). Does anyone have any other
(better) ideas?
Much obliged in advance,
Pat
__________________________
Patrick J. Loll, PhDProfessor of Biochemistry & Molecular BiologyDrexel
University College of MedicineRoom 10-102 New College Building245 N. 15th
St.Philadelphia, PA 19102-1192 USA
(215) 762-7706pj...@drexel.edu
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