Re: [ccp4bb] How to transfer non-frozen crystals with less disturbance?

2014-07-03 Thread meisam nosrati
, Meisam Nosrati wrote: > Dear CCP4ers > > I need to transfer some crystals mainly in sitting drops to the site of > data collection without freezing them. > > I do not know what is the best solution to secure the boxes in their place > to minimize the disturbance. > >

[ccp4bb] How to transfer non-frozen crystals with less disturbance?

2014-07-02 Thread Meisam Nosrati
drops. I have one or two hanging drop boxes as well with 10 microliter size drops. If you have any experience about this matter, I greatly appreciate, if you share it with me. Thanks Meisam Nosrati

Re: [ccp4bb] Refinement with new ligands to PDB

2014-04-01 Thread meisam nosrati
Thank you very much everyone for your comments. I will try your ideas and try to get this thing to work. I appreciate your help Meisam On Mon, Mar 31, 2014 at 7:21 PM, Meisam Nosrati wrote: > Dear CCP4ers > > I have crystallized a protein with a series of ligands that are not in

[ccp4bb] Refinement with new ligands to PDB

2014-03-31 Thread Meisam Nosrati
Dear CCP4ers I have crystallized a protein with a series of ligands that are not in the PDB. I have made the pdb and the torsion libraries in the jligand and given them the new three letter codes, and then I try the refinement in the PHENIX or CCP4, but since these ligands are not in the librar

[ccp4bb] Acceptable Clash Score

2012-11-07 Thread Meisam Nosrati
Dear CCP4ers I want to know what is considered an acceptable Clash Score for a solved structure. Thanks Meisam

Re: [ccp4bb] Problem with making PDB from Coot

2012-07-23 Thread meisam nosrati
appriciate your help Meisam On Mon, Jul 23, 2012 at 5:31 PM, Meisam Nosrati wrote: >