I second Eleanor's suggestions. In fact I would hope the PDB made
re-depisitions known to the original authors. I had an incident recently
where someone (I believe possibly a student) had used one of my structures
for MR but then did the deposition as a re-refinement (because they used it
as MR for
We should also be aware that there is a formal IUPAC definition of hydrogen
bonds as well, although its not really optimized for protein
crystallography.
https://www.iupac.org/publications/pac/pdf/2011/pdf/8308x1637.pdf
Matthew Merski
Univ. of Warsaw
On Thu, Sep 20, 2018 at 5:28 PM Mark Wilson
Hi Alexandre,
I was curious if I could also get a copy of the illustrations if it wont be
too much trouble? I was more curious about 0.0 - 4.0 Ang region if that
saves you some effort.
Thank you for this.
Matthew Merski
On Tue, Nov 28, 2017 at 4:12 PM, Alexandre Ourjoumtsev
gets
overloaded) picture of the geometry.
Matthew Merski
University of Warsaw
On Wed, Jun 14, 2017 at 7:23 PM, Evan Waldron wrote:
> Dear CCP4bb,
>
>I recently solved the 2.0 Å crystal structure of a small hydrophobic
> molecule bound to a protein. A glutamine sidechain nitrogen is pos
reference is
"Data for Biochemical Research" by Dawson, Ch. 18). Acetate also shares
this invariance but the Good buffers often do not. This is of course a
concern with the Spyro Orange experiment as well.
Matthew Merski
Shoichet Group
UCSF
ement
with that product isn't perfect either as the nitrogen has too many
electrons to be in the tertiary center to fit this density.
So, has anyone seen or heard or can imagine a way to get an extra atom on
acetate? Thanks for any help you can give.
Matthew Merski
Post-doc
Shoichet Group
UCSF
Ok, to sum up for the board, a good reference for this problem is at:
http://mcl1.ncifcrf.gov/waugh_tech/faq/tev.pdf
Thanks to everyone who responded.
Matthew
On Mon, May 24, 2010 at 9:27 AM, Matthew Merski <
mer...@blur.compbio.ucsf.edu> wrote:
> Hello all,
>
> I am
ce as the
original proteolytic site, I have been unable to get cleavage despite using
a lot of TEV at 37 C, pH 8.0. Has anyone been able to overcome a similar
problem?
Matthew Merski
Post-doctoral researcher
UCSF
