Ok, to sum up for the board, a good reference for this problem is at: http://mcl1.ncifcrf.gov/waugh_tech/faq/tev.pdf
Thanks to everyone who responded. Matthew On Mon, May 24, 2010 at 9:27 AM, Matthew Merski < mer...@blur.compbio.ucsf.edu> wrote: > Hello all, > > I am working with a protein that is expressed as with an N-terminal domain > that is normally cleaved for activation of the protein (and > crystallization). For in vitro reasons I've needed to switch the normal site > to a TEV site. However, even though the TEV site is in the same place as the > original proteolytic site, I have been unable to get cleavage despite using > a lot of TEV at 37 C, pH 8.0. Has anyone been able to overcome a similar > problem? > > > Matthew Merski > Post-doctoral researcher > UCSF >
