Hi,
Can anyone recommend a good desktop computer for running crystallography
programs? I am looking for a Mac or Linux computer that is relatively fast
and moderately priced. I am not interested in building my own system; I
want a computer that ready to use right out of the box. Any suggestions
Hi,
I am looking for alternative suppliers or similar items for the following
products that were carried by Hampton Research but have since been
discontinued.
1. Cryo vial holder. This is a hexagonal, metal holder that can hold 8
cryo vials. It is handy for freezing multiple crystals at once an
Hello,
We will be setting up a new facility soon and are making a preliminary
purchase list. We may purchase a crystallization robot. I wanted to know
what the estimated (ballpark) price was for either a Mosquito or Art
Robbins Griffin crystallization robot. I have requested quotes, but I was
h
Hello all,
I am working on a ligand binds near the active site of the protein, such
that part of the ligand would clash with part of the natural substrate. I
recently co-crystallized the enzyme with both molecules and solved the
crystal structure to high resolution (around 1.4 angstrom). Surpris
SSLR always returns your dewars containing liquid nitrogen and with
hazardous liquid nitrogen stickers on the dewars. If you are shipping the
dewar dry, you should not need the stickers. If you do not take the
stickers off before re-shipping them, the dewar is likely to be returned.
We had this i
Hello,
I want to use carba-NAD+ (carbanicotinamide adenine dinucleotide), a
non-hydrolyzable analogue of NAD+ for structural and functional studies of
an enzyme. I have seen this compound used in several publications, but the
publications either did not disclose the source of the compound or it w
I recently upgraded to Sierra 10.12.1 and found that none of my
crystallography programs (Coot, ccp4, phenix) worked. I downloaded XQuartz
again and that seemed to fix everything. This is the only time that I
upgraded my computer since probably 2013 or 2012, and the only reason that
I did it was
Hello all,
I am working on a structure in space group P4 at a resolution of about 4
angstrom. Xtriage indicates that translational NCS is present. I am able
to solve the structure by molecular replacement with four copies in the
asymmetric unit, and there are two sets of identical copies. Howev
Hello all,
I was wondering if anyone knew how the RSRZ score was calculated in the
protein data bank validation reports and how useful of a metric this
actually is for structure validation? I am trying to improve this score on
a structure that I am working on, but I'm not really sure where to beg
> *FAX: (517) 353-9334 <%28517%29%20353-9334>
> Email: rmgarav...@gmail.com *
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>
> On May 15, 2014, at 6:50 PM, Matthew Bratkowski
> wrote:
>
> Hello all,
>
>
> I am w
Hello all,
I am working on the structure of a small protein in space group P212121.
The protein is monomeric in solution based on gel filtration analysis.
The Matthews Coefficeint program indicates that 9-10 molecules per
asymmetric unit results in ~50% solvent content, while 1 molecule per
asy
Hi Raji,
I have no experience with membrane proteins, but I have used SUMO tags
frequently. Unlike other proteases that cleave at a specific site
(thrombin, TEV, etc.), Ulp1 recognizes the tertiary structure of SUMO for
cleavage. So if only about 50% of your protein is cleaved, this may
indicate
Hi.
I was considering using GTP Agarose Resin for the final clean up step of the
purification of a GTPase and was wondering if anyone has had experience
using this resin. My main concerns are whether it actually has a decent
binding capacity for GTP binding proteins, considering that endogenous
G
I like using Izit dye from Hampton (
http://hamptonresearch.com/product_detail.aspx?cid=4&sid=41&pid=33) to check
if crystals are protein or salt. If the crystals are protein, the dye
should absorb rather readily into the crystals and turn them blue, while the
rest of the drop will eventually turn
Hi.
Thanks for all of the helpful advice. Below is a summary of the
suggestions, along with some things that I have tried and the results thus
far.
1) Make sure that the crystals are protein and not salt.
My crystals absorb Izit dye well and shooting some initial crystals did not
produce any di
Hi.
Here is some additional information.
1. The purification method that I used included Ni, tag cleavage, and SEC
as a final step. I have tried samples from three different purification
batches that range in purity, and even the batch with the worst purity seems
to produce crystals.
2. The pr
Hello.
I have obtained disk shaped crystals of a protein that I am working on. I
got hits in about 10 different conditions, with a few common precipitants
and pHs, and I have optimized two conditions so far. In the optimized
conditions, the crystals appear overnight, usually surrounded by or hid
It could be caused by iron contamination in one of your buffers. We used to
buy glycerol in a metal canister and metal would leach into the glycerol.
Because of this, one protein that I worked with would turn yellow, even at
relatively low concentrations. I did not have this issue when using
glyc
Hi.
What size are the impurities? If they are smaller than your protein, then
they could actually be truncation products, which will be difficult to
purify away since they maintain some of the same characteristics as the full
length protein. You can check for C-terminal truncations using a
His-a
Hi.
I am working with a protein that has difficulties staying in solution when
concentrated. The buffers that I have been using contain 20 mM Tris pH 8.0,
5 mM BME (or 2 mM DTT), 10% Glycerol, and NaCl from 50 mM up to 2 M. The
protein seems fine to dialyze into low salt buffer (50 mM NaCl) when
Hi.
We have been using His-Select Resin from Sigma in our lab for a number of
years now. When we first bought the resin, I usually got much better purity
of His tagged proteins compared with regular Ni-NTA resin. However, after
regenerating the resin several times, the level of purity seems to h
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