[ccp4bb] how to make a stereo image of electron densities

Dear all, We are preparing a submission to Nature Structural and Molecular Biology, but do not know how to make a stereo image of electron densities as required by the journal. We would appreciate it very much if you give us some detailed protocols on doing that. Thanks! Matt

[ccp4bb] convert map coefficients to phases and FOM

Hi, I am trying to calculate phases and FOM from a model map. However, CNS 1.2 model_map.inp does not output map phases and FOM. Instead, it only gives map coefficients. My question is how to convert these map coefficients to phases and FOM. Does anyone have a script to do that? Thanks so muc

[ccp4bb] low UV reading on AKTA prime

Hi all, We recently got low UV reading on our AKTA prime. We contacted GE healthcare, but was told that the UV reading on AKTA prime should be 20% of the spectrometer reading, because the path length of the flow cell of AKTA prime is only 2 mm (20% of the pathlength of a spectrometer cuvette).

[ccp4bb] expression of single chain variable fragment

Dear all, I'd like to express a single chain variable fragment (scFv) for crystallization purposes, but cannot decide whether I should do it in insect cells (let the antibody be secreted into culture medium) or bacteria (let the antibody be secreted into periplasmic space). If someone could give

[ccp4bb] protocol for harvesting proteins from bacterial periplasmic space

Dear all, I plan to prepare a protein fused to pelB signal peptide and secreted to bacterial periplasmic space. Does anyone have a detailed protocol for harvesting the protein from bacterial periplasmic space? The protein needs to be in the native state and will be purified on a nickel column.

[ccp4bb] distance range of salt bridges

Dear all, I have a question about distance range of salt bridges. I understand that salt bridges within 4 angstroms are considered strong. However, at 5 angstroms or even 6 angstroms, are salt bridges nonexistent or just being weak? Thanks! Matt