Re: [ccp4bb] Crystallization of low solubility proteins from glycerol-containing solutions

Hi Roger, I crystallized a protein that started in solution with 250 mM NaCl, 50% glycerol, and 50 mM Arg+Glu. I initially used traditional microbatch under paraffin oil to get crystallization hits (where protein and screen are 1:1; glycerol ends up at 25%). Then, I found that setting up sittin

Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins

We've been using Bio-Rad biologic duoflow FPLCs for over 5 years (we have 4 of them on site). They can also handle abuse and cold room storage. Their hardware and software are user-friendly. It seems the Profinia system is geared toward simpler purifications.

[ccp4bb] SOLUTION: tcl/tk issue with ccp4 installation 64-bit fedora12

Okay, I got the problem resolved in the following way (thanks go to Clint Leysath): 1. removed the tcltk++ directory that came with my ccp4 download 2. installed Activestate's tcltk 8.4.19.2 from https://www.activestate.com/activetcl/downloads/ 3. downloaded blt2.4z.tar.gz and the blt2.4z-patch-2

[ccp4bb] tcl/tk issue with ccp4 installation 64-bit fedora12

I am grappling with a linux system for the first time. I am successful in compiling the source for ccp4 files. When I start ccp4i I see the normal interface and I can click on the buttons, but the buttons that have menu lists do not respond to clicks. My terminal reports the following: * Applicatio

Re: [ccp4bb] HKL2000 and Fedora 12

Thanks! The 75 dpi fonts fixed it!

[ccp4bb] MODIP

Does anyone know if the program MODIP is still available? Anyone know any other programs that will do what MODIP does? Thanks! Mark

[ccp4bb] Getting a copy of ESCET

I am trying to get a current version of ESCET to compare several models of the same protein with different ligands. I have tried twice to contact Thomas Schneider at the address listed in this post: >Hi, > >for obtaining ESCET, please contact me directly at: > >thomas.schnei...@embl-hamburg.de > >

[ccp4bb] ArpWarp error

I'm running flexWarp on a MacBook Pro and on a Linux box. The program goes through several cycles and then gives the following errors when trying to improve an MR solution: If I start from the PDB file as is ERROR Exception class : 'IndexError' Message : '' BackTrace (limited to level 20) :

[ccp4bb] alternatives to IZIT

Before I place an order for some Izit, are there some other dyes I can use to check if I've got a protein crystal? Thanks, Mark

[ccp4bb] poll: cutoff for "high resolution"

At what refinement resolution or resolution ranges would you call a structure "high resolution" vs. "low resolution"? I realize that this may boil down to semantics (e.g. some may classify structures as "medium resolution"), but I wanted to get an opinion from the pros.