Re: [ccp4bb] cryo-EM

2014-08-29 Thread Marcus Fislage
cedure Best Regards Rana -- Marcus Fislage, PhD Howard Hughes Medical Institute (HHMI) Columbia University Department of Biochemistry and Biophysics Lab of Joachim Frank New York, NY Phone: 212.305.9524 Fax: 212.305.9500

Re: [ccp4bb] CC1/2, XDS and resolution cut off

2012-08-06 Thread Marcus Fislage
Hello > Edward A. Berry wrote: > What about collecting in the corners of a square detector? > Due to the crystal diffracting better than expected or > the need to sacrifice resolution for spot separation? This is actually our reason that we have problem. The strategy initially suggested lower re

[ccp4bb] CC1/2, XDS and resolution cut off

2012-08-06 Thread Marcus Fislage
significant. Are we right to do so or would you disagree? Thanks for any input Marcus -- Marcus Fislage Structural Biology Brussels Vrije Universiteit Brussel Department of Structural Biology, VIB Oefenplein, Gebouw E Pleinlaan 2, 1050 Brussel Belgium Tel: +32-2-629 18 51 Email : marcus.fisl...@vib

[ccp4bb]

2012-04-02 Thread Marcus Fislage
Dear Abraham, after you added all TER signal and have all rows with atom number present you can use pdbset to renumber all atoms. For this take care that you call first your TER signal ATOM (otherwise this row will be deleted). Regards Marcus - Original Message - From: protein chem

[ccp4bb] Refmac: sidechain bond breaks

2011-01-17 Thread Marcus Fislage
Dear all, I might excuse myself for the silly question but it is the first time I solve an x-ray structure. After modell building in coot and running of refmac with restrained refinement I have the problem that the pdb output file contains distances between e.g. ILE Cb and Cg that are so long tha