Hi Rana,
in contrast to Brads answer, and in case I understand you well, Cryo
EM is not in ts infancy stage (as long as you do not talk about Cryo
EM on crystals.
For cryo EM you need only several ul of a nM solution.
I can always suggest you the 3dem bb. There are of course still
limitataions on the size of protein target and resolution, but those
are slowly falling.
Some reading material
http://www.ncbi.nlm.nih.gov/pubmed/23181775
http://www.ncbi.nlm.nih.gov/pubmed/25071206
Cheers
Marcus
Quoting Brad Bennett <bradbennet...@gmail.com>:
Yes, but the technique is still in its infancy stage.
DOI: 10.1016/j.sbi.2014.03.004
Cheers-
Brad
On Fri, Aug 29, 2014 at 6:34 AM, rana ibd <
00000285de88044a-dmarc-requ...@jiscmail.ac.uk> wrote:
Dear CCP4
I wanted to ask has anyone tested 3D protein structure by cryo-electron
microscopy? what is the suitable concentration required for this procedure
Best Regards
Rana
--
Marcus Fislage, PhD
Howard Hughes Medical Institute (HHMI)
Columbia University
Department of Biochemistry and Biophysics
Lab of Joachim Frank
New York, NY
Phone: 212.305.9524
Fax: 212.305.9500
