Your 0.1 M Phosphate/citrate can form crystal at high PEG. Deqian
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yibin Lin
[yyb...@gmail.com]
Sent: Tuesday, February 22, 2011 2:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Could some
It is normal for me, expressed a few proteins before and mass conformed all MET
is SE-met. DQ
From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Yibin Lin
[yyb...@gmail.com]
Sent: Thursday, January 06, 2011 3:06 PM
To: CCP4BB@JISCMAIL.AC.UK
Sub
Agree. two comments for your reference:
1. When you have glycerol in your protein buffer, always add same % glycerol
in your reservoir solution.You have 10% glycerol in your protein buffer, but
not in your reservoir solution, the glycerol will overcome all the other facts
and "grasp" water f
