It is normal for me, expressed a few proteins before and mass conformed all MET is SE-met. DQ ________________________________________ From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Yibin Lin [yyb...@gmail.com] Sent: Thursday, January 06, 2011 3:06 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] selenomethinine-labeled protein
Dear all, I try to express selenomethinine-labeled protein in E.coli methinoine auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18 hours at 37 degree. I don't why the colour of medium vary from colorless to yellow. Could anybody tell me it is normal or not? Thanks a lot! Lin
