Re: [ccp4bb] best linux for laptops?

2009-09-03 Thread Li Sheng
Based on my experience, ubuntu has the most hardware drivers. If your computers are old, they should be supported by most linux distribution versions. CentOS may also be a good choice. Dual boot would not be a problem. From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of

Re: [ccp4bb] Lost my protein

2009-05-05 Thread Li Sheng
Dear Zhang, Perhaps you can try to concentrate your protein using PEG2. Put your protein into dialysis tubing and bury it with PEG2. You should check it every 30-60 min. Sincerely, Li Hi all, during concentrating my protein, using Amicon Ultra centrifugal filter

[ccp4bb] 答复: [ccp4bb] Cryo-protectant

2009-04-23 Thread Li Sheng
Hi Liew, Have you ever tried paratone or some other oil? Sincerely, Li __ Email:s...@sibs.ac.cn Institute of Biochemistry and Cell Biology Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road, Shanghai 2

[ccp4bb] protein purity in SAXS

2009-04-23 Thread Li Sheng
Dear all, Is there anyone with experience in small angle x-ray scattering? Could anyone please tell me the requirements for protein sample purity and concentration? Thanx in advance. Sincerely, Li __ Email:s...@sibs.ac.cn Institute of Biochemistry and Ce

[ccp4bb] setpaths.sh

2007-12-27 Thread Li Sheng
Dear All, I installed ccp4 6.0.2 on my fedora8 platform. It seems I need one extra file named "setpaths.sh", which I can not find in $CCP4/lib/cctbx/cctbx_build/� &� ${CCP4}/src/phaser/phaser-${phaser_version}/build/${phaser_mtype}/ Where can I get such a file? Thanks in advance. Sincerely,

Re: [ccp4bb] [SPAM] [ccp4bb] Problem is creation of Symmetry molecule PDB

2007-12-26 Thread Li Sheng
 me to get dodecameric pdb file? Thanks in advance. Have a nice day!!! Yours sincerely, Sampath Sincerely yours, Li Sheng 2007-12-26 __ Email:[EMAIL

Re: [ccp4bb] advice for crystallizing hydrophobic small molecules

2007-07-18 Thread Li Sheng
quot; > small molecules. > > Thanks in advance- > Todd Green > University of Alabama at Birmingham > . ======== Sincerely yours, Li Sheng 2007-07-19 __ Ema

[ccp4bb] twin?

2007-07-08 Thread Li Sheng
Hi, Dear All, How could I determine whether a crystal was twin or not from its diffraction data? Is there any possibilities that a twin crystal contains P2 and C2 space group? What's the characteristic in such a data file? Thanx in advance. Sincerely, Li Sheng 2007-

[ccp4bb] twin

2007-07-08 Thread Li Sheng
Hi, Dear All, How could I know whether a crystal was twin or not from it's diffraction data? Thanx in advance. Sincerely, Li 07-08-2007