Dear Zhang,
Perhaps you can try to concentrate your protein using PEG20000. Put your protein into dialysis tubing and bury it with PEG20000. You should check it every 30-60 min. Sincerely, Li Hi all, during concentrating my protein, using Amicon Ultra centrifugal filter devices, 5000g, 4C, I lost large amount of my protein (75%). I heard the same story from one of my colleagues too. It seems the membrane of the Amicon tube ate my protein. Why this happen and how to recover my protein? Thank you very much. Yan
