should be submitted as soon as possible,
application review will begin on September 1, 2013. This position
will remain open until filled. The University of Oklahoma is an equal
opportunity institution. www.ou.edu/eoo
Leonard Thomas, Ph.D.
Macromolecular Crystallography Laboratory
available
immediately and will remain
open until filled.
For further information, please contact Dr. Ann West by phone (405)
325-1529 or email
aw...@ou.edu.
The University of Oklahoma is an equal opportunity institution. www.ou.edu/eoo
Leonard Thomas, Ph.D.
Macromolecular Crystallography
Assistant Professor – Structural Biology. The Department of Chemistry
and Biochemistry
(http://chem.ou.edu) at the University of Oklahoma (http://www.ou.edu)
invites applications for
a tenure-track faculty position at the rank of Assistant Professor
beginning August 16, 2013. We
are especia
.
Cheers,
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
lmtho...@ou.edu
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab
asymmetric unit vs. one for the crystal that they were now working
with. Lesson learned make sure your sequence files have the proper
number of copies that you expect.
Cheers,
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of
pdb file only has coordinates in it.
Anybody else run into this problem?
We were able to get a good solution using MolRep and it turns out to
be 2 molecules in the symmetric unit in R32:H. If you input R32:H
into Phaser it blows up.
Regards,
Len
Leonard Thomas Ph.D.
Macromolecular
when going into a separately made well solution. I
will look it trying a number of suggestions given.
Cheers,
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101
crystals crack as soon as they are transfered out of the original
drop. I am running out of ideas and really would love some new ones.
Thanks in advance.
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
nt" alarms available which consist mainly of a strobe
light for the room that it is installed in so you could look into that
if it really is a problem of having the alarms go off for no reasons.
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahom
I have had a fair amount of success using 2.1-2.3 M Na Malonate as a
cryo protectant for crystals from high salt conditions like yours.
Fluorinated oils are also an option, they generally are a little
easier to work with then Paratone.
Good Luck
Leonard Thomas Ph.D.
Macromolecular
Given some of the news reports about the testing of TSA in the US I
would say you might have a good chance of getting the bomb on the
plane. Now if you want more then 3 oz. of mouthwash you could be in
trouble.
Leonard Thomas
lmtho...@ou.edu
On Sep 9, 2009, at 6:18 PM, Ezra Peisach
the glycerol
concentration very gradually. I had a colleague who said the protein
he worked on in grad school had to be stepped up 1% increments or it
would crack and fall apart. IIRC he had better then 2.0 ang. data for
the final structure.
Leonard Thomas Ph.D.
Macromolecular
The scratch directory was there but it was owned by root for some
reason. After deleting it and rerunning the set up scripts all works
fine.
Cheers,
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620
seems to be ok. I have some users who
are just learning and I really don't think they get much out of the
learning process watching me on my personal computer run through things.
Cheers,
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Okl
.
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73019
lmtho...@ou.edu
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
On May 20, 2009, at 12:32 PM, Seema Mittal
be an a gray area from my perspective, the the density seems
to indicate alternate conformations.
Cheers,
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73019
lmtho...@ou.edu
Just an update, I ended up downloading binaries per Axel, and
everything appears to be happy.
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73019
lmtho...@ou.edu
http
+0x11ca): In function `vopen_':
: undefined reference to `rename_'
collect2: ld returned 1 exit status
problems with new executable - old version retained
This is a fresh install on this machine. Any ideas would be
appreciated.
Thanks,
Leonard Thomas Ph. D.
Macromolecular Crystallography
. Of course these are probably more the exception
then the rule.
Len
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73032
lmtho...@ou.edu
Office: 405-325-1126
Lab: 405-325
Why not just use a 96 well plate. Should be able to do this by hand
with a large enough drop. You can get them from either TTP Labtech or
Grace Biosystems, I think that is the name.
Cheers,
Len
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
puzzling over it and have tried a number of things to see if
the structure is biologically significant.
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73032
lmtho...@ou.edu
Hello All,
A grad student here is working on a structure, actually a series of
structures, that have in interesting feature that we are not sure how
to handle. She has taken a know protein and linked the N and C
terminal ends varying the linkage from being direct to a 10 residue
linker.
I have run across a problem and I am not sure where it originates
from. When I use a pdb input file that contains a metal atom, say
Cu, that has been written out by coot in refmac I get a read error of
the sort that refmac does not recognize the Cu. If I then edit the
pdb file and change
I have a Shelx HKLF4 formated reflection file that I would like to
convert to a mtz file. The primary reason is to transfer the freeR
set. I found a couple of convoluted ways of converting the
reflection data, but no real way of getting the freeR set to transfer.
Any suggestions?
Cheers
Hello,
Personally I don't really care if I have a stereo setup or not. I
have not really used stereo to build in probably 3-4 years. The
majority of the users of my facility on the other hand really want
stereo. Usually in the initial and final stages of building and
analysis. It is
The first thing to try before going down the long road of fussing
with cryo is to take a shot at room temp. and see how your crystal
diffracts in general. It is true it may be a cryo problem, but if
the non cryo protected crystals do not diffract then why would one
expect the cryo protecte
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